Modifying Enzymes
New England Biolabs, Inc. EpiMark Hot Start Taq DNA Polymerase – 100 reactions
EpiMark Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45C, but releases the enzyme during normal PCR cycling conditions. This permits assembly of reactions at room temperature. An advantage of the aptamer-based hot start mechanism is that it does not require a separate high temperature incubation step to activate the enzyme. The advanced aptamer-based hot-start activity coupled with the supplied optimized reaction buffer makes the EpiMark Hot Start Taq DNA Polymerase an excellent choice for use on bisulfite-converted DNA. Taq DNA polymerase possesses a 5- 3 polymerase activity (1,2,3) and a 5 flap endonuclease activity (4,5). EpiMark Hot Start Taq DNA Polymerase is supplied with 5X EpiMark Hot Start Taq Reaction Buffer, providing robust amplification for bisulfite-converted DNA and AT-rich amplicons.
New England Biolabs, Inc. T4 PDG (T4 Endonuclease V) – 2000 units
T4 PDG (pyrimidine dimer glycosylase) has both DNA glycosylase and AP lyase activity. The 16 kd protein recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. The enzyme cleaves the glycosyl bond of the 5' end of the pyrimidine dimer and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.
BioVendor, LLC PROTEIN LYSOSTAPHIN 5 MG
TypeRecombinant proteinDescriptionLysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.SourceE. coliPurity97.5% as determined by RP-HPLC.Biological ActivityDetermined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30°C.FormulationThe protein was lyophilized without any additives.Physical AppearanceSterile filtered white lyophilized (freeze-dried) powder.
Zymo Research Corporation dsDNA Shearase™ Plus 50 U (1 U/ul)
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
Enzo Life Sciences Thapsigargin, (1mg), CAS Number: 67526-95-8
ATPase inhibitor. Formula: C34H50O12. MW: 650.8. CAS Number: 67526-95-8. MI: 14: 9272. Purity: ≥95% (HPLC). Appearance: Colorless wax or white solid. Solubility: Soluble in DMSO (20mg/ml), 100% ethanol (20mg/ml) or acetone. Use/Stability: Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Long Term Storage: -20°C
Worthington Biochemical Corporation CHYMOTRYPSIN ALPHA TLCK 100MG
Chymotrypsin, alpha, TLCK treated, Storage: 2degree to 8degreeC, Source: Bovine pancreas, Pack Size: 100mg.
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 10000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
New England Biolabs, Inc. GpC Methyltransferase (M.CviPI) – 200 units
Methylates all cytosine residues (C5) within the double-stranded dinucleotide recognition sequence 5´...GC...3´.
New England Biolabs, Inc. Endoproteinase LysC – 20 µg
Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.
Sigma Aldrich Fine Chemicals Biosciences Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 50ML
Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 50ML
Abcam Recombinant Human Liver Carboxylesterase 1/CES1 protein, 10UG
Recombinant Human Liver Carboxylesterase 1/CES1 protein is a Human Fragment protein, in the 19 to 562 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, HPLC.
Protein/ Peptide/ Enzyme Synonyms: CES2, SES1, CES1, Liver carboxylesterase 1, Acyl-coenzyme A:cholesterol acyltransferase, Brain carboxylesterase hBr1, Carboxylesterase 1, Cholesteryl ester hydrolase, Cocaine carboxylesterase, Egasyn, HMSE, Methylumbelliferyl-acetate deacetylase 1, Monocyte/macrophage serine esterase, Retinyl ester hydrolase, Serine esterase 1, Triacylglycerol hydrolase, ACAT, CE-1, hCE-1, CEH, REH, TGH
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
Inulinase from Aspergillus niger lyophilized, powder, brown-gray, ~25 U/mg | 9025-67-6 | MFCD00217463 | 250MG
United States Biological Corporation ZYMOLYASE 100T 25MG
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg
United States Biological Corporation ZYMOLYASE 100T 50MG
Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg